Bpd, Cell Culture - Cambridge, United Kingdom - AstraZeneca

AstraZeneca

Verified Company

Cambridge, United Kingdom

4 weeks ago

Posted by:

Tom O´Connor

beBee Recruiter

Description

Early Expression and Supply (EES) - Part of Cell Culture & Fermentation Sciences (CCFS)

Description of work / project to be undertaken:

The Early Expression and Supply team is responsible for generating therapeutic proteins, usually antibodies, to support in-vitro and in-vivo studies at early project stages.

For the rapid production of these therapeutic proteins, AZ has developed a proprietary high-yielding, scalable and easy to use Chinese Hamster Ovary (CHO) cell based transient expression system.

This process is used at small scale (shake flasks) to assess expressibility of the proteins and at larger scales L rocking bioreactors) for material generation.

The team is constantly looking at ways to improve the process further focusing in the following areas:

optimisation of plasmid DNA production (in microbial systems), optimisation of the transfection conditions, assessment of impact of key process parameters, such us dissolved oxygen (DO) and pH on cell performance and protein yield, and finally on the robustness and scalability of the process in both rocking and stirred-tank reactors.

At the end of the internship the student will gain experience in microbial and CHO cell culture, plasmid DNA production, transient expression in bioreactors, experimental design, data analysis and in-depth understanding of biopharmaceutical development.

Primary duties of this position:

Gain experience in:

Attend and engage in department / team meetings, with opportunity to present
Plan and execute experiments, including relevant literature reviews. Analyse data and writeup experiments in electronic lab notebook.
Present findings, summarise outcomes and share future recommendations.

What are we looking for?
At AstraZeneca, we put patients first and strive to meet their unmet needs worldwide. We're deeply committed to our strategy of being a great place to work. For us, that includes fostering an environment where everyone can be among inclusive, supportive individuals with curious minds.

A company that genuinely follows the science and values individuals at all levels, you'll be backed and encouraged to speak up, ask questions, and share ideas to push the boundaries of science and continuously learn and explore.

Our purpose is bold and so is our approach.

Becoming a more agile and innovative company means building a dynamic, inspiring culture where we celebrate entrepreneurial thinking and act with a sense of urgency.

We are courageous, taking risks and learning from both success and failure. We are curious, creative, and open to new ideas and ways of working. Above all, we are passionate about science and driven to always put patients first.

When you join us, you will be part of a great place to work; in an environment that energizes and empowers each of us to achieve our goal to develop and deliver medicines.