Research Associate - White City, United Kingdom - Autolus

Autolus
Autolus
Verified Company
White City, United Kingdom

1 week ago

Tom O´Connor

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Tom O´Connor

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Description

Location:


  • White City, London, UK
    Job Summary:

Role Summary


We are looking for a motivated Research Associate level scientist to join a multidisciplinary group of researchers within the Binder Discovery Group.

This role will focus generation and characterisation of new antibody-based proteins targeting a variety of cancer antigens, TME factors and cell engineering targets to develop and improve CAR T cell therapy.


This work will be performed in close collaboration with the wider Autolus R&D teams and successful applicants will work within a wider context of translational and cell manufacturing divisions.

Rapid translation to clinical testing is a key motivation of the company. This is a unique opportunity to work at the forefront of immunotherapy in rapidly moving and truly multi-disciplinary field.


Key Responsibilities
***- Antibody generation and protein engineering to target oncology focused antigens.

  • Mammalian cell culture techniques including aseptic work with multiple different cell lines and culture types.
  • Molecular biology techniques including PCR, cloning, DNA analysis, modelling of cloning using appropriate software (snapgene etc).
  • Characterisation of antibody and CAR T cell performance including;
  • Protein analyses techniques; Purification, ELISA, Biacore SPR, SEC chromatography, DSF etc.
  • Cell analysis techniques; Cell culture, flow cytometry, Incucyte, cytokine assays, cytotoxicity assays.
  • Clear and concise documentation of laboratory findings in electronic lab notebook.
  • Plan and execute experiments with conflicting timelines.
  • Interpret complex data sets using statistics packages and present conclusions concisely including in presentations to R&D teams and VP/CSO.
  • Any other duties as required following consultation with the post holder.

Demonstrated skills and competencies

E - Essential

P - Preferred

Experience - Prior evidence of substantial laboratory-based experience is a prerequisite.

  • Knowledge and experience of working with display systems for antibody discovery, including but not limited to phage display (P), as well as yeast and mammalian display (P).
  • Understanding of antibody and CAR structures, and the current state of the art for CAR T cell therapy. (E)
  • Experience in mammalian cell culture (E), transfection and viral manufacture and transduction. (P)
  • Performance and analysis of large NGS data sets for antibody discovery. (P)
  • Highly proficient using standard molecular and synthetic biology techniques as well as protein production, purification and analysis methods. (E)

Qualifications
MSc or equivalent in Biology/Biochemistry or related discipline and demonstrable lab experience. Applicants with substantial (3-4 years) relevant industrial experience but not advanced degrees are also welcome to apply. (E)


Skills/Specialist knowledge

  • Familiarity with B cell and T cell biology and immunology (E).
  • Understanding of oncology, therapeutic modalities and the advantages/ difficulties facing the field. (E)
  • Understanding of CAR T cell therapy and antibodybased therapeutics. (E)
  • Strong skills in molecular cloning including construct and library design, PCR, RT-PCR, DNA assembly and E. coli transformation. (E)
  • Familiarity with DNA analysis software such as Snapgene. (P)
  • Familiarity with protein production and purification (E), and assays such as ELISA, flow cytometry, and proteinprotein interaction analysis (BIAcore). (P)
  • Strong verbal and written communication skills. (E)
  • High attention to detail. (E)
  • Be a selfstarter and willing to learn new skills. (E)
  • Commitment to high quality work.(E)
  • Ability for creative and critical thinking. (E)
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