Postdoctoral Research Assistant - Dundee, United Kingdom - University of Dundee

University of Dundee

Verified Company

Dundee, United Kingdom

2 weeks ago

Posted by:

Tom O´Connor

beBee Recruiter

Description

Postdoctoral Research Assistant - MRC PPU:

Employer
University of Dundee
Location
Dundee, Dundee City (GB)
Salary
£36,024
£44,263 pa
Closing date
7 Jun 2024
Discipline

Chemistry
Job Type
Postdoctoral, Research Assistant
Employment - Hours
Full time
Duration
Fixed term
Qualification
PhD
Sector
Academia- You need to sign in or create an account to a job.

Chemical Biology - Discovery Probes for Ubiquitin-like E3 Ligases

Modification of proteins with ubiquitin-like proteins (Ubls) is a critical cellular process linked to pathogen resistance, cancer, and selective organelle degradation.

Central to their conjugation to substrates are E3 ligase enzymes (E3s), yet for many Ubls the E3s are poorly understood or remain completely unknown.

The Virdee lab has pioneered the development of activity-based probes (ABPs) for the related ubiquitin system, which has led to several field-changing discoveries (Pao et al., Nat Chem Bio 2016; Pao et al., Nature 2018; Mathur et al., Cell Chem Biol.

2020; Mabbitt et al. Nat Chem Biol This project will build upon this expertise and expand its approach to Ubl systems. Ubl E3 ABPs will be constructed using the lab's modular chemical protein synthesis methods. These powerful tools will then be deployed in combination with proteomics to discover entirely novel E3 ligases.

Insights into these understudied posttranslational modifications will enrich our understanding of their function and could enable the development of new therapeutic targets.

Your priorities will include:

Chemical protein synthesis of proteinbased probe molecules for Ubl E3s
Assays using purified protein and cell extracts
Preparation of data for manuscript preparation
Liaise with other lab members and collaborators
Maintenance of meticulous lab records

Who we're looking for:

Enthusiasm for technology development working at the chemistrybiology interface
Experience with chemical biology techniques
Biochemical experience

This contract is for a fixed term period of 3 years.

MRC Protein Phosphorylation and Ubiquitylation Unit (MRC PPU):

School of Life Sciences (SLS):

The MRC PPU is based within the School of Life Sciences at the University of Dundee, a world-class academic institution with a reputation for the excellence of its research, its high-quality teaching and student experience, and the strong impact of its activities outside academia.

With 900 staff from over 60 countries worldwide the School provides a dynamic, multi-national, collegiate and diverse environment with state-of-the-art laboratory, technology and teaching facilities.