Research Associate, Genome Editing - Berkeley, United Kingdom - Springer Nature

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Description

Research Associate, Genome Editing (9612C), Innovative Genomics Institute :

Employer
University of California, Berkeley
Location
Berkeley, California (US)
Salary
DOE
Closing date
29 Mar 2024
Discipline

Life Science
Job Type
Researcher
Employment - Hours
Full time
Duration
Permanent
Qualification
Undergraduate
Sector
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Research Associate, Genome Editing (9612C), Innovative Genomics Institute
About Berkeley

At the University of California, Berkeley, we are committed to creating a community that fosters equity of experience and opportunity, and ensures that students, faculty, and staff of all backgrounds feel safe, welcome and included.

Our culture of openness, freedom and belonging make it a special place for students, faculty and staff.

The University of California, Berkeley, is one of the world's leading institutions of higher education, distinguished by its combination of internationally recognized academic and research excellence; the transformative opportunity it provides to a large and diverse student body; its public mission and commitment to equity and social justice; and its roots in the California experience, animated by such values as innovation, questioning the status quo, and respect for the environment and nature.

Since its founding in 1868, Berkeley has fueled a perpetual renaissance, generating unparalleled intellectual, economic and social value in California, the United States and the world.

Departmental Overview

The Innovative Genomics Institute (IGI) is a non-profit, academic research organization formed through the partnership of UC Berkeley and UC San Francisco, and located on the UC Berkeley campus.

A key focus of the IGI is accelerating translation of CRISPR-based approaches to address real-world problems in human health, agriculture, and climate.

Application Review Date

The First Review Date for this job is: January 30, 2024

Responsibilities:

Perform recombinant DNA procedures, including via use of laboratory automation hardware, to construct novel expression vectors for genome editors.
Characterize the vectors by sequencing and perform mRNA in vitro transcription for subsequent incell testing.

Recombinant DNA:

Routine plasmid maintenance and sequencecharacterization, performing a variety of cloning procedures such as Gibson cloning; scaleup to laboratory automation (with assistance from automation specialist.
Design and execution of PCR and sequencebased assays to qualitycontrol resulting expression constructs.

Nucleic acid-directed assays:

Design and execution of a variety of assays (eg based on NGS or ddPCR) aimed at detecting genome editing outcomes.

Mammalian cell culture:

Human transformed tissue culture cell line passaging and transfection.
Manage miscellaneous molecular techniques such as RT-PCR, ddPCR, and NGS.
Assist personnel in nonclinical lead teams with design and testing of novel constructs and molecular assays to assess genome editing potency and specificity.
Perform quality control of genome editor compositions by delivery to human tissue culture cells, genomic DNA harvest, and nextgeneration sequencing followed by data analysis.
Analyze experimental data and assist Beacon researchers in preparing scientific presentations and reports.
Maintain laboratory supplies, protocols, and hardware for consistent operational readiness to meet project goals.

Required Qualifications