Research Scientist - Cambridge, United Kingdom - Reed Engineering

Reed Engineering

Verified Company

Cambridge, United Kingdom

3 weeks ago

Posted by:

Tom O´Connor

beBee Recruiter

Description

Scientist/Research Scientist - Protein Engineering

Permanent, Full-Time

Cambridge area, UK

REED Scientific are working with an international biopharmaceutical company who discover, design, and develop some of the most innovative and effective medicines for patients worldwid
e.

About the Job

They are searching for a molecular biologist to join the Protein Engineering group working on GPCR structure determination to enable drug discovery efforts.

In this role you will be responsible for:

Cloning of plasmid constructs for expression;
Expression of GPCRs in insect, yeast and human cells;
Characterization of GPCRs in membranes and in detergentsolubilised form using ligand binding assays;
Establishing and optimising thermal stability assays;
Mutant library generation and directed evolution of proteins;
Screening for stabilising mutations using our proprietary platform to create StaR proteins;
Construct engineering to optimise expression;
Purification and biochemical characterisation of GPCRs using a range of techniques such as size exclusion chromatography;
Optimising expression of other membrane proteins (e.g., ion channels and transporters)
Careful documentation of experimental work; and,
Work closely with molecular biologists, protein expression scientists, biophysicists, structural biologists, and pharmacologists to help advance the project.

Skills/experience:

BSc or MSc in a relevant area of biology
Ability to work flexibly within a team, whilst being highly selfmotivated and able to work independently;
Analytical thinking and good problemsolving skills;
Excellent organisational and timemanagement skills, including the ability to work to strict deadlines;
Fluency in oral and written English is essential; and
Relevant lab experience would be preferred including:
Experience in cloning techniques;
Proficient in cell culture techniques for the expression of proteins in mammalian, insect or yeast cells;
Experience in protein purification;
Experience of assay development and optimisation;
Experience in flow cytometry;
Experience in GPCR _in vitro_ pharmacology and GPCR biochemical assays would be desirable.

This is a one-of-a-kind opportunity to join a burgeoning company, and work using state-of-the-art scientific equipment and facilities.

If this sounds of interest and you meet the requirements, don't miss this opportunity