James Daubney

am a highly skilled, dynamic, committed, and motivated senior scientist with over 8 years of experience in the field of drug discovery, responsible for the identification of a lead candidate drug (EXN407) and subsequent successful phase Ib/IIa clinical trial, looking for new opportunities in the field of drug discovery and life sciences research. I have extensive experience in novel assay design, ADME and pharmacokinetics, formulation development and drug discovery. I am currently looking for a change in career with a particular interest in pathology. 

Employment History 

Senior Scientist at Exonate Ltd: January 2022 – June 2024 

Scientist at Exonate Ltd: November 2015 – December 2022 

Successfully took company from start-up stage to identification and discovery of lead candidate drug. Key roles in extensive screening, assaying novel pharmacological targets, formulation and drug development. Identified drug candidate for successful clinical phase Ib/IIa clinical trial. 

Sandwich course Biomedical Science Histopathologist in QMC with experience in clinical routine histopathology and post mortem tissue work. 

Publications 

Daubney J, Bonner PL, Hargreaves AJ and Dickenson JM (2015) Cardioprotective and cardiotoxic effects of quercetin and two of its in vivo metabolites on differentiated H9c2 cardiomyocytes, Basic & Clinical Pharmacology & Toxicology, 116, 96–109  

Research and technical skills 

Bioluminescence resonance energy transfer (NanoBRET)  

Certified LC-MS/MS hardware and software operator: Shimadzu 8050 triple quad MS.  

Cellular thermal shift assay (CETSA) for drug target evaluation. 

Rapid equilibrium dialysis (RED) for plasma protein, serum, tissue and melanin binding.  

Amplification, purification & transfections: plasmid DNA (Electroporation and Fugene). 

Drug solubility and stability measurement. 

Ex vivo porcine and human ocular drug permeability assay.  

Dissection of human, non-human primate, pig, rabbit and rat tissues. 

Routine human clinical histopathological dissection & special staining techniques. 

Trans-Scleral/trans-dermal drug formulation and preparation. 

Culture and cryopreservation of primary and immortalised mammalian cell lines. 

Cytotoxicity, apoptosis, differentiation, ROS/oxidative stress and cell viability assays. 

Protein purification and estimation. 

ELISA. 

Cell signalling and reporter assays. 

IT and data management skills 

Dotmatics R&D scientific database and Vortex visualisation and data-mining software. 

Shimadzu Lab solutions and insight Mass spectrometry control and analysis software. 

Analysis and Statistics (GraphPad Prism). 

Licor Image and Empiria studio.   

2D-gel analysis (Progenesis SameSpot). 

FACS analysis (Kaluza Flow Analysis Software). 

Skilled in MS office suite software. 

Ph. D. in recognition of research titled “The Cardioprotective effects of dietary Flavonoids”, Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham Trent University (January 2011 – July 2015). 

First Class Honours Bsc (Hons) Applied Biomedical Science, School of Biomedical and Natural sciences, Nottingham Trent University (October 2007 – July 2010).  

A-level Biology, Chemistry and Physics (2005-2007) Bilborough College 

GCSE Double Science (A) French (A) Religious Studies (A) History (B) English & English Lit (B), Business studies (B), Electronics (B) Maths (B)