Nayab Fatima

Versatile skilled individual with transferrable skills from working at management level in academic research pharmaceutical industry, customer service and sales. I have managed multiple start up projects to establish a pipeline for SARS-Cov-2 testing (for NHS and Amazon Diagnostics). Worked at GMP/GCP/GLP lab for quality control management and approval of multiple clinical trials studies, making sure a successful initiation, planning, execution, monitoring, and completion of a clinical trial study. 

Currently I a working with Novel Cryo-ET project of working with genetically engineered mice, fresh living and post-mortem human brain tissue to determine the in-situ 3D molecular structures of amyloid proteins that cause neurodegenerative disease. 

Specialties: Neuroscience, Drug Delivery, Biomaterials, Microbiology, Cryo-Electron Microscopy, Single particle, Negative staining, Protein Purification, TEM, SEM, 2D & 3D cell culture, HPLC, Western blots, EMSA, ELISA, Leadership, Project management, Presentation, People skills, Good listener, Team working, Reliable and Resilient

Postdoc Research Fellow- UKRI future leader fellowship, University of Leeds

I work with genetically engineered mice, fresh living and post-mortem human brain tissue to determine the in-situ 3D molecular structure of amyloid proteins that cause neurodegenerative disease using Cryo-ET and single particle. 

Additionally, my experiments include wet lab work to such as Ex vivo protein purification of fibrils using sarkosyl, performing western blots and Immunohistochemistry as well as negative staining using electron microscope.

The living human brain tissue is received by a collaboration with Mr. Ryan Mathews (Neurosurgeon) at the Leeds General infirmary hospital and from the national institute of Neuroscience hospital (UCL). The Post-mortem tissue received from Netherlands Brain Bank.

I also have home office personal license for animal handling, giving general anesthesia and performing surgeries on small animals (PIL A, B & C). I have trained many (undergraduates and MSc students) with number of experiments.

Doctor of Philosophy (Ph.D) – Neuroscience (Gene drug delivery across CNS using gold nanoparticles)

Department of LHCS, Walton Hall, Milton Keynes, MK7 6AA                                                   Graduated 2020

The research involved attaching therapeutic DNA molecules onto glyco-gold nanoparticles and characterizing using AKTA-FPLC, EMSA and western blotting techniques. Different length DNA attached nanoparticles were tested on human brain 2D (hCMEC/d3) and ON a novel model of 3D co-culture (prepared using iPSCs and collagen gel embedded with glial cells on transwells). The gold nanoparticles in cell cultures were fixed in blocks by resin embedding and then micro sectioned using a diamond knife and quantified using TEM. Additionally, immunohistochemistry with antibodies was used to mark glial cells and imaged on fluorescent microscope. Flow cytometer used for cell cytotoxicity assay. A large amount of in vivo work was regulated by injecting DNA attached nanoparticles into animals and quantifying gold concentration in different organs using ICP/MS while cell localization was observed using histology and microscopic analysis and qPCR was used to quantify DNA in different organs.