Vedant Kolekar

A driven biotechnologist with over 5 years of combined academic, professional
and voluntary experience applying expertise in genetic engineering, protein
characterization, biomolecule conjugation, quality control, and project
management to drive advancements in biopharma. Seeking a Scientist role
leveraging multifaceted research background spanning mass spectrometry,
HPLC, genomics, immunology, and bioinformatics to contribute to cutting-edge
drug development and address complex healthcare challenges. Known
for technical skills in assay optimization, regulatory compliance,
troubleshooting, and attention to detail to ensure research integrity and
product quality. With a passion for innovation and proven ability to lead high-performing
teams, ready to utilize analytical capabilities and collaborative
mindset to maximize research productivity in service of advancing patient
health through pioneering biotechnology.

Personable and strong determined research scientist with experience in diagnostic reagent development and immunology. Analytical problem-solver comfortable working in fast-paced environments with minimal oversight. Self-motivated, driven, and displays initiative to prioritize tasks and, handle numerous assignments simultaneously. Innovative thinker and efficient in designing experimental approaches in the field of pharmacology, therapeutics, and biologics. Ability to work in cross-functional culture to give optimum results and achieve definite goals.

Accomplishments:
•Research and operations planning for quantitative analysis of HbA1c.
•Performed clinical conjugations for biomarkers and tracers to study intracellular interactions and monitor cell kinetics.
•Validation and documentation control for various technological transfers overseas. 
•Developed protocols for aseptic techniques in molecular biotechnology involving culturing and production of various proteins using fermentation technology.
•Extraction and mapping of genomic variation in the bacterial cell using various plasmid retention techniques, reverse genetics and gene probe analysis.
•Developed protocols for unknown protein purification to study protein characterization.
•Troubleshoot various heterogeneous assays for clinical parameters by using ELISA, CLIA and FLIA techniques.
•Developmental research of electrochemistry compounds useful for studying the protein redox interaction for energy transfer kinetics.
•Designing a CRISPR variant project on Bananas as an idea for a market product for the next decade.
•Reviewing and critical analysis of the paper on Receptor gene technology used for monitoring the modification of potato
•Communicative project and presentation for ideology on funding and scope in the biotechnology market.

University of Birmingham (UOB), Birmingham, UK
MSc in Molecular Biotechnology, 2022-2023
Coursework: Research techniques in Molecular Biotechnology, Functional Genomics and Reverse Genetics, Pharmaceutical and Therapeutic Biologics, Practical approach and experimental design, Funding and Communicating science, Data analysis and interpretation.
Thesis: Characterization of Phycobiliproteins in Cyanobacteria using Mass Spectroscopy | MAY 2023- SEPT 2023
Domain – Mass Spectroscopy, Protein characterization, Electrospray Ionization, Cell harvesting & purification
Description – Culture spirulina to harvest biliprotiens and study the change of relative abundance between phycocyanin and glutathionylated phycocyanin by protein extraction and mass sprectrometry. Developed methodology to attain oxidative stress to produce glutathionylated phycocyanin and purified using AKTA